LC-IMPACT 2.0 quantifies the impacts of blue water consumption on freshwater biodiversity at the damage level. Impacts are expressed as regional and global PDF·yr per cubic meter of water consumed. The method links water consumption to reductions in river discharge (fate factor) and the resulting loss of freshwater fish species richness (effect factor). Unlike earlier approaches assuming a one-to-one relationship, the updated model captures complex hydrological exchanges between rivers, groundwater, soil, and the atmosphere. The river basin is used as the native spatial scale. Fate factors are derived from a coupled surface water–groundwater model, comparing natural and human-impacted conditions over several decades. Effect factors rely on an enhanced Species–Discharge Relationship (SDR+), which explains fish richness using discharge, elevation, climate zones, and basin characteristics. This enables near-global applicability and better representation of regional ecological differences. Global extinction probabilities convert potentially reversible regional losses into global biodiversity damage. Both marginal and average characterization factors are provided to support small-scale and large-scale assessments.