Land use is a main driver of global biodiversity loss (MES 2005). Within a product’s life cycle, the land use impacts can represent a significant portion of their total environmental burden. The impact assessment approach proposed by Chaudhary et al. (2015) using countryside species-area relationship (SAR) is used for calculating ecoregion specific marginal and average characterization factors (CFs) for biodiversity loss for both land occupation and transformation.